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  4. A comprehensive, cell specific microRNA catalogue of human peripheral blood
 
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2017
Journal Article
Title

A comprehensive, cell specific microRNA catalogue of human peripheral blood

Abstract
With this study, we provide a comprehensive reference dataset of detailed miRNA expression profiles from seven types of human peripheral blood cells (NK cells, B lymphocytes, cytotoxic T lymphocytes, T helper cells,monocytes, neutrophils and erythrocytes), serum, exosomes and whole blood. The peripheral blood cells from buffy coats were typed and sorted using FACS/MACS. The overall dataset was generated from 450 small RNA libraries using high-throughput sequencing. By employing a comprehensive bioinformatics and statistical analysis, we show that 3' trimming modifications as well as composition of 3' added non-templated nucleotides are distributed in a lineage-specific manner-the closer the hematopoietic progenitors are, the higher their similarities in sequence variation of the 3' end. Furthermore, we define the blood cell-specific miRNA and isomiR expression patterns and identify novel cell type specific miRNA candidates. The study provides the most comprehensive cont ribution to date towards a complete miRNA catalogue of human peripheral blood, which can be used as a reference for future studies. The dataset has been deposited in GEO and also can be explored interactively following this link: http://134.245.63.235/ikmb-tools/bloodmiRs.
Author(s)
Juzenas, S.
Venkatesh, G.
Hübenthal, M.
Hoeppner, M.P.
Du, Z.G.
Paulsen, M.
Rosenstiel, P.
Senger, P.
Hofmann-Apitius, M.
Keller, A.
Kupcinskas, L.
Franke, A.
Hemmrich-Stanisak, G.
Journal
Nucleic Acids Research  
Open Access
DOI
10.1093/nar/gkx706
Link
Link
Language
English
Fraunhofer-Institut für Algorithmen und Wissenschaftliches Rechnen SCAI  
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