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  4. The molecular blueprint of a fungus by next-generation sequencing (NGS)
 
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2017
Book Article
Title

The molecular blueprint of a fungus by next-generation sequencing (NGS)

Abstract
Sequencing the whole genome of an organism is invaluable for its comprehensive molecular characterization and has been drastically facilitated by the advent of high-throughput sequencing techniques. Especially in clinical microbiology the impact of sequenced strains increases as resistance and virulence markers can easily be detected. Here, we describe a combined approach for sequencing a fungal genome and transcriptome from initial nucleic acid isolation through the generation of ready-to-load DNA libraries for the Illumina platform and the final step of genome assembly with subsequent gene annotation.
Author(s)
Grumaz, Christian  
Kirstahler, Philipp  
Sohn, Kai  
Mainwork
Human fungal pathogen identification. Methods and protocols  
DOI
10.1007/978-1-4939-6515-1_21
Language
English
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
Keyword(s)
  • next-generation sequencing (NGS)

  • whole-genome shotgun sequencing (WGS)

  • RNA sequencing (RNA-seq)

  • Fungal genome

  • assembly

  • annotation

  • Bioinformatics

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