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1991
Conference Paper
Title
Morphogenesis and tropism of organoid vascular pattern in tissue and on interfaces in vitro.
Other Title
Morphogenese und Tropismus von organoiden vaskulären Mustern in Gewebe und auf Grenzflächen in vitro
Abstract
Vascular morphogenesis, the formation of organoid capillary patterns may result from the interaction of monocytes (macrophages) and endothelial cells. Monocytic morphogen activities for endothelial cells were purified (>100'000 fold) to homogeneity and characterized in structure and function from supernatants of serum-free cultures of isolated, lectin-activated porcine peripheral monocytes and from ischemic/infarcted (inflamed) heart muscle sites prepared by transient coronary occlusion by transfemoral catheterization. An isolated monokine (angiotropin) acts as morphogen in vivo and in vitro in the femto mol range: In tissues of different species, bioactive vascular patterns are induced by sprouting and capillarization phases with vascular leaky tips in transition; a relevance of the reactions in pathophysiological phenomena is suggested and may be exemplified by transient neo-hypervascularization of skeletal muscle (rabbit) with reversible, long-lasting increase in hemodynamics as vis ualized by high 99 Tc-angioscintigrams; as corollary, on defined surfaces, in cultures of cloned endothelial cells, it induces selectively cellular differentiation, migration and spatial organization basic to in-vitro-angiogenesis (1-4). As possible differentiation makers in phenotype changes of endothelial cells, the enzymes D-xylose: NADP 1-oxidoreductase (E.C. 1.1.1.179), polyol: NADP oxidoreductases (aldose reductases, E.C. 1.1.1.21), aldehyde: NAD oxidoreductases (E.C. 1.1.1.3), aldose 1-epimerase (E.C. 5.1.3.3) and carbonate hydro-lyase (carbonic anhydrase, E.C. 4.2.1.1) were investigated (5). The morphogen structure as bioactive copper-ribonucleo-polypeptide complex (Cu-RNP) was determined by its chemical and enzymatic disintegration and modification by HPLC and micro-detection methods (1). Proteolysis of polypeptide and removal of complexing Cu-ion components resulted a RNA (75 bases) (6,7). The RNA was isolated and subjected to chemical sequence analysis. It was found peculia r
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