Microencapsulation of insulin and its release using w/o/w double emulsion method
The present work is devoted to design of capsules with hydrophilic drugs such as insulin to preserve its stability and biological activity using the double emulsion technique and its subsequent entrapment into biodegradable microcapsules with shells made of Chitosan and Xanthan Gum complexes. Formulation parameters (type of biopolymer, type of oil and stabilizer and its concentration, volume fraction and content of internal aqueous phase, time and regime of mixing) were investigated.The effect of the complexes on the process of emulsion formation and on the stability and characteristics of the resulting emulsions was investigated by measurements of size distribution (DLS) and interfacial charge (Zeta-potential). The resulting capsules were characterized by microscopy, size distribution and Zeta-potential measurements. The release kinetics of insulin was monitored using UV-vis spectroscopy and showed the progressive enhancement of sustainability.