Fluorescence spectroscopy by detection of glucose concentrations in DMEM-solutions and its perspectives for non-invasive measurement

An easy accessible and low-cost method for glucose concentration monitoring and diabetes management will be a great help for more than 250 millions of diabetic patients worldwide to avoid the risks and the complications caused by hyper- or hypoglycemia. This paper shows the results obtained using fluorescence spectroscopy for detecting the glucose concentrations in DMEM solutions. By irradiating DMEM solutions that have different glucose concentrations with light, a few wavelengths in UV- and visible-range for the calculations of glucose concentrations using fluorescence spectroscopy are applied and the detected signals were analyzed. For the detection of glucose concentration noninvasively using various optical methods, the interaction between light and definite glucose solutions was studied. The developed compact system will enable the application of different LASER diodes (LD' s) or light emitting diodes (LED's) in the range of UV and NIR in an easy manner. Variable intensity, frequency and duty cycle can be adjusted for fluorescence and other optical measurements. A multi-sensor taking all perturbations into account will be a good choice for glucose monitoring. Fluorescence measurements at wavelengths below 800 nm and especially the measurements at the wavelength 485 nm give reproducible glucose concentrations results from DMEM glucose solutions at a constant temperature.