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  4. Tuning the surface of nanoparticles: Impact of poly(2-ethyl-2-oxazoline) on protein adsorption in serum and cellular uptake
 
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2016
Journal Article
Title

Tuning the surface of nanoparticles: Impact of poly(2-ethyl-2-oxazoline) on protein adsorption in serum and cellular uptake

Abstract
Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.
Author(s)
Koshkina, Olga
Westmeier, Dana
Lang, Thomas
Bantz, Christoph  
Hahlbrock, Angelina
Würth, Christian
Resch-Genger, Ute
Braun, Ulrike
Weise, Christoph
Thiermann, Raphael  
Eravci, Murat
Mohr, Benjamin
Schlaad, Helmut
Stauber, Roland H.
Docter, Dominic
Bertin, Annabelle
Maskos, Michael  
Journal
Macromolecular bioscience  
DOI
10.1002/mabi.201600074
Language
English
ICT-IMM  
Keyword(s)
  • cellular uptake

  • nanoparticles

  • poly(2-ethyl-2-oxazoline)

  • poly(ethylene glycol)

  • protein adsorption

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