Structure analysis of carboxymethyl starch by capillary electrophoresis and enzymic degradation
Carboxymethyl starches (CMS) with a degree of substitution (DS) in the range of 1.2 to 1.5 were analysed by capillary electrophoresis (CE) after hydrolysis and reductive amination in a borate buffer. The monomer composition determined was compared to data calculated by the statistical models of Spurlin and Reuben. In addition, the starch derivatives were exhaustively degraded by alpha-amylase and amyloglucosiclase and the amount of glucose liberated was determined. Results were discussed with regard to derivatisation conditions and properties of the carboxymethyl starches.