• English
  • Deutsch
  • Log In
    Password Login
    Research Outputs
    Fundings & Projects
    Researchers
    Institutes
    Statistics
Repository logo
Fraunhofer-Gesellschaft
  1. Home
  2. Fraunhofer-Gesellschaft
  3. Artikel
  4. A miniaturized ISFET-ELISA system with a pretreated fused silica capillary as reaction cartridge
 
  • Details
  • Full
Options
1996
Journal Article
Title

A miniaturized ISFET-ELISA system with a pretreated fused silica capillary as reaction cartridge

Abstract
A universal ELISA-based analytical system is described, which consists of a flow-through set-up with a pretreated fused silica capillary as a reaction cartridge and an ISFET array as a detector. The reliability of the device has been tested for two different analyses: T-2 toxin and saxitoxin. For each toxin a reversible indirect competitive ELISA has been established that uses an activated fused silica capillary as a solid phase. Urease, which acts as enzyme label in the immunoassay, produces a pH-shift that can be detected by the ISFET array. Due to a preincubation step the lower detection limit is 10 pg/ml for T-2 toxin. The main advantages of the system are small sample volumes, short cycle times and an excellent stability of the regenerable receptor layer.
Author(s)
Yacoub-George, E.
Wolf, H.
Koch, S.
Woias, P.
Journal
Sensors and Actuators. B  
DOI
10.1016/S0925-4005(97)80016-0
Language
English
IFT  
  • Cookie settings
  • Imprint
  • Privacy policy
  • Api
  • Contact
© 2024