Kinetics of human neutrophil surface marker expression and chemotaxis during granulocyte colony-stimulating factor treatment

We previously reported an altered surface marker expression and chemotaxis of G-CSF-induced neutrophils from patients with severe congenital neutropenia. However, effects of G-CSF and the influence of the underlying disease on neutrophils could not be distinguished from one another. In this study, we evaluated the effects of G-CSF on neutrophil phenotype and function in patients under chemotherapy and in healthy test subjects. We found a significantly enhanced expression of Fc(gamma)RI, CD14, and CD54 and a decrease in the level of Fc(gamma)RIII. In addition, motility of G-CSF-induced neutrophils was significantly decreased. Neutrophil phenotype and migration were altered only during G-CSF therapy. The effects were seen in patients under cytotoxic chemotherapy and in healthy test subjects. Surface marker alterations and neutrophil motility were affected by G-CSF in a dosedependent manner. (Abstract truncated)