Chrondrogenic predifferentiation of human mesenchymal stem cells in collagen type I hydrogels
Hyaline cartilage displays a limited regenerative potential. Consequently, therapeutic approaches have been developed to treat focal cartilage lesions. Largersized lesions are commonly treated by osteochondral grafting/mosaicplasty, autologaus chondrocyte implantation (ACI) or matrix-induced chondrocyte implantation (MACI). As an alternative cell source to chondrocytes, multipotent mesenchymal stem cells (MSCs) are regarded a promising option. We therefore investigated the feasibility of predifferentiating human MSCs incorporated in hydrogels clinieally applied for MACI (CaReS@). MSCladen hydrogels were cast and cultured over 10 days in a defined chandrogenie differentiation medium supplemented with TGF-ß1. This was followed by an 11-day culture in TGF-ß1 free media. After 21 days, considerable contraction of the hydrogels was observed. Histochemistry showed cells of a chondrocyte-like morphology embedded in a proteoglycan-rieh extracellular matrix. Real-time polymerase chain reaction (RT-PCR) analysis showed the expression of chandrogenie marker genes, such as collagen type II and aggrecan. In summary, we dernarrstrate that chandrogenie differentiation of human mesenchymal stem cells embedded in collagen type Ihydrogels can be induced under the influence of TGF-ß1 over a period of 10 days.
Universitätsklinikum Hamburg-Eppendorf / Klinik und Poliklinik für Unfall-, Hand- und Wiederherstellungschirurgie
Reichert, Johannes C.
Evangelisches Waldkrankenhaus Spandau / Klinik für Orthopädie und Unfallchirurgie