Development approach of a workflow for 3D printing of living mycelium materials and their growth manipulation

As a viscous mycelium-substrate-composite, mycelia can be processed into more complex geometries in generative extrusion-based processes, even without molding tools. Biological transformation can also affect the properties of a printed object such that the mycelium still lives and grows after the 3D-build-process and changes the object over time. Depending on the substrate composition, type of fungus and growth conditions, different properties of the object can be manipulated. Different test geometries with corresponding preliminary characterization tests are shown. The present results demonstrate that test geometries could be successfully 3D printed with living mycelium material and manipulated during cultivation.