Generation of ready-to-use SPR chips using automated nano-spotting

Microarray technology and protein biochips have advanced a lot in the past years and have allowed for substantial progress in bioanalysis and biosensors, especially in the fields of proteomics, protein quantification and functional protein evaluation. In combination with surface plasmon resonance (SPR)-based biosensors they have become important research tools for the real-time detection of molecular interactions, especially without having to resort to labeling. SPR offers numerous advantages over alternative methods, including rapid and real-time detection, high sensitivity and low sample consumption, and has been widely adopted both in basic research and in industry. In this paper we describe the functionalization of Cytomegalovirus pp65 antigen on SPR chips utilizing a nanoplotter robotic platform for the preparation of microarrays with up to 138 spots.