The determination of trifluoroacetic acid in rat milk samples by 19F-NMR spectroscopy and capillary gas chromatography

The 19F-NMR spectroscopy provides a specific method for the identification and quantification of TFA in biological matrices. [...] In our investigation, calibration curves were obtained by integrating 19F-NMR signals of the spiked rat milk samples and plotting the peak against the known concentration of TFA in the standard samples. Then, the peak areas of the corresponding 19F-NMR signal in the study samples were interpolated against these calibration curves to calculate the TFA concentrations present. In this way, recovery experiments were not necessary. [...] (Citated from 3. Results and discussion)