Biomineralization and remineralizing potential of toothpastes containing nanosized β-calcium glycerophosphate: an in vitro study

To evaluate the efect of 1100 ppm F toothpastes supplemented with micrometric or nanosized β-CaGP (β-CaGPm/β-CaGPn) on artifcial enamel remineralization, using a pH cycling model. Enamel blocks with artifcial caries were randomly allocated into ten groups (n=10), according to the toothpastes: without fuoride/β-CaGPm/β-CaGPn (negative control); 1100 ppm F (1100F); 1100F plus 0.125%, 0.25%, 0.5%, and 1.0% of β-CaGPm or β-CaGPn. The blocks were treated 2×/day with slurries of toothpastes. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); integrated mineral loss (ΔIMR); fuoride (F), calcium (Ca), and phosphorus (P) concentrations in the enamel; polydispersity index (PdI); and zeta potential (Zp) were determined. The data were analyzed by ANOVA (p<0.001). For Zp/PdI, no signifcance was observed when comparing the means (p>0.001). The treatment with 1100F-0.25%β-CaGPn led to %SHR ∼57 higher when compared to the 1100F group (p<0.001). The lowest ΔKHN was observed for the 1100F-0.25%β CaGPn group (p<0.001). The ΔIMR was lower (∼201%) for the 1100F-0.25%β-CaGPn when compared to 1100F (p<0.001). The association of β-CaGPm and β-CaGPn to 1100F did not infuence its F concentration (p>0.001). The highest increase in Ca and P was observed for 1100F-0.25%β-CaGPn (p<0.001). The addition of 0.25%β-CaGPn to 1100F toothpaste wasTo evaluate the efect of 1100 ppm F toothpastes supplemented with micrometric or nanosized β-CaGP (β-CaGPm/β-CaGPn) on artifcial enamel remineralization, using a pH cycling model. Enamel blocks with artifcial caries were randomly allocated into ten groups (n=10), according to the toothpastes: without fuoride/β-CaGPm/β-CaGPn (negative control); 1100 ppm F (1100F); 1100F plus 0.125%, 0.25%, 0.5%, and 1.0% of β-CaGPm or β-CaGPn. The blocks were treated 2×/day with slurries of toothpastes. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); integrated mineral loss (ΔIMR); fuoride (F), calcium (Ca), and phosphorus (P) concentrations in the enamel; polydispersity index (PdI); and zeta potential (Zp) were determined. The data were analyzed by ANOVA (p<0.001). For Zp/PdI, no signifcance was observed when comparing the means (p>0.001). The treatment with 1100F-0.25%β-CaGPn led to %SHR ∼57 higher when compared to the 1100F group (p<0.001). The lowest ΔKHN was observed for the 1100F-0.25%β CaGPn group (p<0.001). The ΔIMR was lower (∼201%) for the 1100F-0.25%β-CaGPn when compared to 1100F (p<0.001). The association of β-CaGPm and β-CaGPn to 1100F did not infuence its F concentration (p>0.001). The highest increase in Ca and P was observed for 1100F-0.25%β-CaGPn (p<0.001). The addition of 0.25%β-CaGPn to 1100F toothpaste wasTo evaluate the efect of 1100 ppm F toothpastes supplemented with micrometric or nanosized β-CaGP (β-CaGPm/β-CaGPn) on artifcial enamel remineralization, using a pH cycling model. Enamel blocks with artifcial caries were randomly allocated into ten groups (n=10), according to the toothpastes: without fuoride/β-CaGPm/β-CaGPn (negative control); 1100 ppm F (1100F); 1100F plus 0.125%, 0.25%, 0.5%, and 1.0% of β-CaGPm or β-CaGPn. The blocks were treated 2×/day with slurries of toothpastes. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); integrated mineral loss (ΔIMR); fuoride (F), calcium (Ca), and phosphorus (P) concentrations in the enamel; polydispersity index (PdI); and zeta potential (Zp) were determined. The data were analyzed by ANOVA (p<0.001). For Zp/PdI, no signifcance was observed when comparing the means (p>0.001). The treatment with 1100F-0.25%β-CaGPn led to %SHR ∼57 higher when compared to the 1100F group (p<0.001). The lowest ΔKHN was observed for the 1100F-0.25%β CaGPn group (p<0.001). The ΔIMR was lower (∼201%) for the 1100F-0.25%β-CaGPn when compared to 1100F (p<0.001).
The association of β-CaGPm and β-CaGPn to 1100F did not infuence its F concentration (p>0.001). The highest increase in Ca and P was observed for 1100F-0.25%β-CaGPn (p<0.001). The addition of 0.25%β-CaGPn to 1100F toothpaste was able to promote an additional remineralizing effect of artificial caries lesions.