Focal cerebral ischemia by permanent middle cerebral artery occlusion in sheep

According to the recommendation of international expert committees, large animal stroke models are demanded for preclinical research. Based on a brief introduction to the ovine cranial anatomy, a sheep model of permanent middle cerebral artery occlusion (MCAO) will be described in this chapter. The model was particularly designed to verify several therapeutic strategies during both, acute and long-term studies, but is also feasible for development of diagnostic procedures. Further, exemplary application of imaging procedures and imaging data analyses using magnetic resonance imaging (MRI) and positron emission tomography (PET) are described. The chapter also includes recommendations for appropriate animal housing and medication.