Towards a new therapy concept for acute microbial karatides, including Acanthamoebae

Purpose The purpose of this study was to develop novel therapeutic approaches for the management and treatment of microbial keratitides, especially pathologies resulting from Acantamoeba castellanii inoculations. Methods Growth inhibitory effects of plasma-induced reactive species on cultures of Staphylococcus aureus, Pseudomonas aeruginosa, Acantamoeba castellanii, and MRSA were assayed for in in an in-vitro setting. Human donor corneas were inoculated and cultured with cultures of above pathogens, followed by staining of cells and microscopic analysis. Results Our results show a substantial inhibitory effect of plasma-induced reactive species on the growth and migration of the pathogens tested. Conclusions The effects observed provide foundation for the development of new avenues for the management and therapy of microbial keratitides.