Optimized creation of monolayers for parallel readout of bead-based asseays

This publication presents the basic studies and the design optimization process of a microfluidic aggregation chamber in order to achieve highly periodical and mechanically stable monolayers of beads. All investigated designs of microfluidic devices exhibit one inlet channel connected to the so-called aggregation chamber. This chamber measures a depth which only slightly exceeds the diameter of the beads and possesses several outlet channels. With depths of the outlets smaller than a bead diameter, the beads are kept back and forced to accumulate inside the aggregation chamber as a monolayer. CFD-simulations are carried out to describe the process of filling and to assist the process of design optimization.