Energy regeneration systems in cell-free protein synthesis in vitro

Cell-free protein synthesis is a rapidly evolving technology that presents an alternative to conventional recombinant protein production. The technology utilizes cell lysates for ex situ protein synthesis. Advantages of the technology include the reduction of energy investments during cell cultivation and also expenses needed for strain optimization. At the moment, cell-free protein synthesis is done predominantly at lab scale in the form of batch processes. Any technology that would allow continuous protein production at commercial scale still needs to be developed. Basic considerations at this point include the design of suitable bioreactors as well as the supply of energy for maintaining the synthesis process. Despite these challenges, investigations show that the implementation of a continuous cell-free protein synthesis process is possible. The exclusive license for this PDF is limited to personal website use only. No part of this digital document may be reproduced, stored in a retrieval system or transmitted commercially in any form or by any means. The publisher has taken reasonable care in the preparation of this digital document, but makes no expressed or implied warranty of any kind and assumes no responsibility for any errors or omissions. No liability is assumed for incidental or consequential damages in connection with or arising out of information contained herein. This digital document is sold with the clear understanding that the publisher is not engaged in rendering legal, medical or any other professional services.