A continuous enzyme membrane reactor retaining the native nicotinamide cofactor NAD-H.

It was shown that high retentions for the native coenzyme NAD(H) may be obtained in a membrane reactor utilizing a negatively charged ultrafiltration membrane. The degree of retention of the total coenzyme (NAD+ and NADH) depends on f e. This ratio is a complex function of the kinetic properties of both enzymes (Km values for the substrate; product inhibition; enzyme stability) and of the operating conditions (absolute concentrations of substrates and products in the reactor). The efficacy of this approach, then, depends on whether a regenerating enzyme may found that possesses favorable kinetic properties.