Synthesis, molecular docking studies, and in vitro screening of sulfanilamide-thiourea hybrids as antimicrobial and urease inhibitors

A series of novel hybrid molecules containing sulfanilamide and thiourea templates was designed and synthesized. These compounds were screened for antimicrobial and urease inhibitory activities. Some of the compounds revealed promising antibacterial and antifungal activities. Fascinatingly, the majority of the compounds exhibited potential urease inhibitory activities with IC 50 ranging from 0.20 to 7.50 µM. Compound 2b was identified as the most potent urease inhibitor (IC50 0.20 µM), and was 100-fold more potent than thiourea, the standard inhibitor. Molecular docking studies of compounds were performed on 3D crystal structures of Jack bean and Helicobacter pylori ureases.