Verfahren zur Untersuchung der Wirkung eines gasfoermigen Mediums auf ein biologisches Pruefsystem unter Verwendung eines extrazellulaeren Metabolisierungssystems sowie Vorrichtung zur Durchfuehrung des Verfahrens

(A1) Die Erfindung betrifft ein Verfahren zur Untersuchung der Wirkung eines gasfoermigen Mediums auf ein biologisches Pruefsystem unter Verwendung eines extrazellulaeren Metabolisierungssystems, umfassend folgende Verfahrensschritte: - Anzuechtung eines biologischen Pruefsystem auf einem permeablen Traeger - Leiten des gasfoermigen Mediums ueber die Oberflaeche des biologischen Pruefsystems zur Bildung einer Expositionsatmosphaere oberhalb des biologischen Pruefsystems - Zugabe des extrazellulaeren Metabolisierungssystems zu einem Erhaltungsmedium - Positionierung des Erhaltungsmediums mit extrazellulaerem Metabolisierungssystem unterhalb des permeablen Traegers mit Kontakt zum permeablen Traeger, derart, dass das extrazellulaere Metabolisierungssystem nur durch den permeablen Traeger durchtritt, das biologische Pruefsystem jedoch nicht vom Erhaltungsmedium mit extrazellulaerem Metabolisierungssystem ueberschwemmt wird.

DE 102007030413 A1 UPAB: 20090222 NOVELTY - The method for investigating the effect of a gaseous medium on a biological test system (28) using an extracellular-metabolizing system, comprises examining the biological test system on a permeable support (30), guiding the gaseous medium over a surface of the biological test system for the formation of an exposure atmosphere above the biological test system, adding the extracellular-metabolizing system to a maintenance medium, and positioning the maintenance medium with the extracellular-metabolizing system below the permeable support. DETAILED DESCRIPTION - The method for investigating the effect of a gaseous medium on a biological test system (28) using an extracellular-metabolizing system, comprises examining the biological test system on a permeable support (30), guiding the gaseous medium over a surface of the biological test system for the formation of an exposure atmosphere above the biological test system, adding the extracellular-metabolizing system to a maintenance medium, positioning the maintenance medium with the extracellular-metabolizing system below the permeable support, adjusting the depression of the extracellular-metabolizing system admitted to the maintenance medium through the permeable support by giving a determined pressure affecting on the maintenance medium with the metabolizing system, and regulating the evaporation rate over the culture by giving a determined temperature in direct surrounding region of the biological test system and/or giving a determined flow rate of the gaseous medium flowing over the surface of the biological test system. The pressure effected on the maintenance medium is selected, so that the metabolizing system depresses through the permeable support and the biological test system does not inundate from the maintenance medium with the metabolizing system. An INDEPENDENT CLAIM is included for an exposure device for investigating the effect of a gaseous medium on a biological test system using an extracellular-metabolizing system. USE - Method for investigating the effect of a gaseous medium on a biological test system using an extracellular-metabolizing system useful for exposing the biological test system in cigarette smoke or in automobile- or industrial exhaust gases, for investigating the environmental atmospheres, work-station atmospheres or inner-space atmospheres, for investigating the effect of a gaseous medium on a biological test system in the field of product security, consumer protection, pharmaceutical development, production supervision or medicine technology, for investigating the effect of a gaseous medium on a biological test system under the observance of organization for economic cooperation and development (OECD) guidelines, and for investigating toxicological effects, genotoxic effects, immunomodulatory or immunotoxic effects or others biological or toxicological relevant cellular changes (all claimed). ADVANTAGE - The method is capable of effectively, reliably and simply investigating the effect of the gaseous medium on the biological test system with high sensitivity and selectivity thus reducing the complexity of cultural practices, and capable of regulating and controlling the flow rate of the gaseous medium on the biological test system.