Method of evaluating at least one image of at least one micro-array

A computer-implemented method of evaluating at least one image (110) of at least one micro-array (112) on a substrate (114), specifically of at least one microscopic fluorescence image (116) of the micro-array (112), the method comprising:i. at least one retrieving step, comprising retrieving the at least one image (110) and retrieving at least one set of deposition data comprising information on a deposition of spots (120) of the micro-array (112) on the substrate (114);ii. at least one pattern recognition step for automatically detecting a printing pattern (130) of the micro-array (112) in the image (110), comprisingii.1. generating a set of test patterns (132) comprising a plurality of test patterns (132), by using the set of deposition data,ii.2. comparing the set of test patterns (132) with information retrieved from the image (110), andii.3. generating an estimate printing pattern (148) in accordance with a degree of congruence of the test patterns (132) with the image (110), the estimate printing pattern (148) having a pattern of grid points (150);iii. at least one dividing step, comprising automatically selecting sub-images (164) of the image (110) in accordance with the grid points (150) of the estimate printing pattern (148); andiv. at least one segmentation step, comprising automatically segmenting spots (120) of the printing pattern (130) in the sub-images (164).Further disclosed are an image evaluation system (208) for evaluating the at least one image (110) of the at least one micro-array (112) and a computer program and a computer-readable storage medium for performing the method.