Verfahren und Vorrichtung zum Bestimmen von Proteinen auf einem Reaktionstraeger

Die vorliegende Erfindung betrifft ein Verfahren zum Nachweisen der Anwesenheit bzw. Abwesenheit eines Proteins/Polypeptids auf einem Reaktionstraeger sowie eine Vorrichtung hierfuer. Dabei sind insbesondere Reaktionstraeger betroffen, die eine Vielzahl von Messpunkten enthalten wie Biochips. Der Nachweis der Proteine erfolgt anhand ihrer Eigenfluoreszenz.

EP 1281969 A UPAB: 20030522 NOVELTY - To determine the presence or absence of at least one polypeptide/protein, at least at one measurement point (10) on a reaction carrier, the sample is exposed to an electromagnetic radiation as excitation light, to excite the intrinsic fluorescence of the polypeptide/protein. DETAILED DESCRIPTION - To determine the presence or absence of at least one polypeptide/protein, at least at one measurement point (10) on a reaction carrier, the sample is exposed to an electromagnetic radiation as excitation light, to excite the intrinsic fluorescence of the polypeptide/protein. The reaction carrier is a biochip (1), with recessed wells as a micro-titration plate. The light has a wavelength of 270-290 nm, and preferably 280 nm, using a laser. The fluorescence is measured by an optical detector. USE - The technique is for use in the analysis of proteins. ADVANTAGE - The proteins can be analyzed using chip technology, with a high sensitivity, in a simple operation, with high local resolution of the proteins to be studied in the native conformation.