Verfahren zur Herstellung von Interferon-beta und deren Varianten

(A1) Die vorliegende Erfindung betrifft Verfahren zur Kultivierung von Interferon-?-produzierenden Zellen sowie zur Gewinnung von Interferon-?-Proteinen oder Varianten davon.

DE 102008051574 A1 UPAB: 20100428 NOVELTY - Extracting a protein with interferon (IFN)- beta -activity comprises: (a) providing cells with the ability to synthesize a protein with IFN- beta -activity and a culture medium containing a block copolymer of ethylene oxide and propylene oxide; (b) culturing the cells in a culture medium under conditions that allow the expression of the protein and its secretion into the culture medium; and (c) obtaining the protein with IFN- beta -activity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for obtaining a purified protein with IFN- beta -activity from a protein containing medium, comprising subjecting the protein containing medium to (i) affinity chromatography, subsequently (ii) a hydrophobic interaction chromatography and subsequently (iii) gel filtration. USE - The method is useful for extracting a protein with IFN- beta -activity. ADVANTAGE - The method produces a protein with IFN- beta -activity in a pure form, preferably with a purity of 99.9%, preferably without using serum albumin, preferably without using human or bovine serum albumin.