Verfahren zur Isolierung von Erythrozyten

Die Erfindung betrifft ein verbessertes Verfahren zur Isolierung interessierender Bestandteile aus biologischen Proben. Dabei werden die interessierenden Bestandteile an Makromolekuelen reversibel gebunden.

DE 19912120 A UPAB: 20001117 NOVELTY - Isolation of erythrocytes (E) comprising treating a sample with at least one substance (I) that promotes aggregation of (E), and separating the aggregates, is new. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a device for isolation of (E) comprising at least one (I) bound to a carrier. USE - The method is used to eliminate (E) from samples before polymerase chain reaction (where (E) act as inhibitors of chain extension), and to recover (E) for subsequent analysis. ADVANTAGE - The method is simpler and less expensive than conventional methods that require centrifugation or specific antibodies. No non-specific binding of leukocytes, viruses and bacteria to (I) occurs, so these do not become incorporated into the aggregates. (E) are reversibly bound to (I) and do not undergo hemolysis.