Dreidimensionales Hautmodell

Verfahren zur Vermehrung von dermalen Fibroblasten, wobei die dermalen Fibroblasten eingebettet in einer dreidimensionalen, gelartigen Biomatrix, enthaltend 3,5 bis 4,5 mg/ml Kollagen in gepuffertem serumhaltigen Zellkulturmedium, wobei die fuer die Herstellung der Biomatrix verwendete Kollagenloesung einen Anteil von .ge. 90 % an nicht-denaturiertem, nativem Kollagen enthaelt, kultiviert werden.

WO 200192477 A UPAB: 20051216 NOVELTY - Method for propagating dermal fibroblasts (A) by culturing them while embedded in a three-dimensional, gel-like biomaterial (B) that contains at least 3 mg/ml collagen, in a buffered, serum-containing culture medium. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for the following: (1) testing function, morphology and/or differentiation status of (A) by growing them as above and simultaneously, or subsequently, testing them; (2) preparing an in vitro dermis equivalent (C) by growing (A) as above then recovering (C) formed; (3) preparing an in vitro skin equivalent (D) by growing (A) as above, then seeding with keratinocytes; (4) (C) and (D) produced as above; and (5) determining the effect of a chemical or other agent on dermis or skin cells by determining its interaction with (C) or (D). USE - The method is used to produce (human) dermis and skin equivalents in vitro. These are used to test effects of chemicals or other agents (e.g. potential pharmaceuticals, cosmetics, heat and light) on human skin cells, especially to detect any irritation, toxic, inflammatory or allergic effects, also to study penetration of substances through skin and to evaluate mechanisms involved in skin diseases (for development of new therapies). ADVANTAGE - The method produces in vitro models that are closely similar (both histologically and functionally) to native human skin. The models include a multilayered epidermis component and have not been subjected to a poorly defined shrinkage step.