Geltraeger zur Durchfuehrung des COMET-Assays mit Automatisierungsmoeglichkeit im Roboter-gestuetzten Analysebetrieb zur Realisierung eines Hochdurchsatz-Verfahrens

(A1) Die Erfindung betrifft einen Geltraeger zur Durchfuehrung des COMET-Assays mit Automatisierungsmoeglichkeit im Roboter-gestuetzten Analysebetrieb zur Realisierung eines Hochdurchsatz-Verfahrens, umfassend eine Traegerplatte, deren Plattenoberseite mindestens eine Traegerflaeche zum Aufbringen des Gels aufweist, wobei die Traegerflaeche einen Haftbereich umfasst, der derart ausgebildet ist, dass das auf der Traegerflaeche aufgebrachte Gel in diesem Bereich an der Plattenoberseite haftet, und einen vom Haftbereich verschiedenen Analysenbereich, der derart ausgebildet ist, dass eine Analyse in diesem Bereich durchfuehrbar ist, wobei sich Haftbereich und Analysenbereich durch physikalische und/oder chemische Eigenschaften in bzw. auf der Plattenoberseite voneinander unterscheiden.

DE 102009039869 A1 UPAB: 20110331 NOVELTY - The gel carrier for implementing comet assay (single cell gel electrophoresis assay) with automation possibilities in robot-based analysis process for the realization of a high throughput method, comprises a carrier plate (12), whose top side (14) has a support surface for applying the gel, where the support surface comprises an adhesive area (18), which is formed in such a way that the gel applied on the support surface adheres in the area at the top of the plate, and an analysis area (20) that is different from the adhesive area. DETAILED DESCRIPTION - The gel carrier for implementing comet assay (single cell gel electrophoresis assay) with automation possibilities in robot-based analysis process for the realization of a high throughput method, comprises a carrier plate (12), whose top side (14) has a support surface for applying the gel, where the support surface comprises an adhesive area (18), which is formed in such a way that the gel applied on the support surface adheres in the area at the top of the plate, and an analysis area (20) that is different from the adhesive area. The analysis area is formed so that the analysis is carried out in the analysis area. The adhesive area and the analysis area differ from one another through physical and/or chemical characteristics in and/or on the top surface. The adhesive area partially surrounding the analysis area is partially surrounded by a hydrophobic region (22) that is coated by a coating on the top surface. The hydrophobic region is applied by screen-printing or tampon printing on the top of the plate. The adhesive area consists of three circle segments or four square segments. The external-facing side of the segment is arc-shaped and internal-facing side of the segment is straight. The analysis area is formed in quadratic or rectangular or circular manner. The support surface comprises two-dimensioned and/or formed adhesive areas. The adhesive area completely or partially covers external circumference of the hydrophobic region under opening of the adhesive area and analysis area. The hydrophobic region is black or white color. USE - Gel carrier useful for implementing comet assay with automation possibilities in robot-based analysis process for the realization of a high throughput method. ADVANTAGE - The gel carrier is inexpensive and conveniently reusable.