Vorrichtung und Verfahren zur Analyse biologischer Proben

(B3) Die Erfindung liegt im technischen Gebiet der Zellbiologie und Transplantatemedizin. Sie betrifft Vorrichtungen und Verfahren zur schnellen und nicht-invasiven Analyse oder Kontrolle biologischer Proben, besonders zur Sterilitaetskontrolle, zur Charakterisierung von in der biologischen Probe enthaltenen infektioesen Partikeln und Mikroorganismen sowie zur Charakterisierung von Gewebezellen und Transplantaten. Hauptanwendungsgebiete der Erfindung sind die biotechnologische Produktion pharmakologischer Wirkstoffe und Therapeutika sowie die Transplantationsmedizin.

DE 102006053540 B3 UPAB: 20080421 NOVELTY - Optospectroscopic analysis of a biological sample in fluids, comprises: fixing the biological sample in a sample holding unit; categorizing the particles that are optionally contained in the biological sample based on an analysis of the image data from the microscopic photographs of the biological sample and/or the particles-contained in it, and analyzing the biological sample within a measurement zone that can be positioned in the region of the biological sample, by Raman spectroscopic method. DETAILED DESCRIPTION - Optospectroscopic analysis of a biological sample in fluids, comprises: fixing the biological sample in a sample holding unit; categorizing the particles that are optionally contained in the biological sample based on an analysis of the image data from the microscopic photographs of the biological sample and/or the particles-contained in it, and analyzing the biological sample within a measurement zone that can be positioned in the region of the biological sample, by Raman spectroscopic method. Based on the results of the categorization procedure, the measurement zone is automatically positioned at defined sections or particles of the biological sample and the Raman spectroscopic analysis is confined to the region of this measurement zone. The fixing of the biological sample takes place by allowing the biological sample to flow through the sample holding unit, which is designed as a fluid cell, where the particles optionally contained in the biological sample are retained in the fluid cell and are fixed there reversibly, at least for the duration of the analysis. The particles are retained in the fluid cell by at least a single perforated plate with many holes that is arranged in the fluid cell and the holes in the perforated plate are arranged in rows and columns and structured in the form of a regular and implementable pattern. An INDEPENDENT CLAIM is included for a device for optospectroscopic analysis of biological samples, comprising: sample holding unit for the local fixation of the biological sample in a measurement zone; excitation radiation unit, suitable for excitation of Raman scattered radiation by excitation radiation in an excitation zone within the measuring zone; and analysis unit, suitable for the spectral analysis of the Raman scattered radiation emitted by the biological sample in the excitation zone, where: the sample holding unit is a fluid cell that can be flowed through by the biological sample; the fluid cell exhibits an instrument that are suitable for retention and temporary reversible fixation of the particles contained in the biological sample; the fluid cell exhibits at least a first perforated plate with several holes with a first diameter as the retention part for holding back the particles, and the holes in the perforated plate are arranged in the perforated plate in rows and columns and structured in the form of a regular and implementable pattern. USE - The method is useful for: the non-invasive, non-destructive control of biological fluids with regards to their sterility and contamination; the non-invasive, non-destructive characterization or typing and/or selection of a biological cell contained in a fluid; the non-invasive, non-destructive characterization of the differentiation status of a biological cell contained in a fluid; the non-invasive, non-destructive characterization or typing of tissues or tissue sections; the non-invasive, non-destructive characterization or typing of allogenous or autologous implants (claimed). ADVANTAGE - The optospectroscopic analysis of a biological sample in fluids is fast, non-destructive and non-invasive and shows a high level of specificity and sensitivity.