Mikrofluidische Anordnung zur Detektion von in Proben enthaltenen chemischen, biochemischen Molekülen und/oder Partikeln

(A1) Die Erfindung betrifft eine mikrofluidische Anordnung zur Detektion von in Proben enthaltenen chemischen, biochemischen Molekülen und/oder Partikeln. Die Detektion kann dabei bevorzugt optisch/massesensitiv durchgeführt werden. Aufgabe der Erfindung ist es, das Anbindungsverhalten von zu detektierenden Molekülen und/oder Partikeln an Fängermoleküle oder Fängersubstanzen zu verbessern. Bei der Erfindung wird in einer mikrofluidischen Anordnung eine flüssige, chemische, biochemische und/oder Partikel enthaltende Probe durch mindestens einen Messkanal in strömender Form geführt. Am Boden eines Messkanals sind dann spezifische Fängermoleküle und/oder Fängersubstanzen immobilisiert, über die die flüssige Probe strömt. Zusätzlich sind aber innerhalb des Messkanals Barriereelemente vorhanden. Die Barriereelemente sind dabei so angeordnet und ausgerichtet, dass sie zumindest bei einem Teil der strömenden flüssigen Probe die Strömungsrichtung verändern. So kann ein Teil der Probe den Messkanal über seine gesamte Laenge im Wesentlichen nicht parallel zur Längsachse des Messkanals durchströmen. Dadurch verlängern sich der dabei zurückzulegende Weg und die Verweilzeit beim Durchströmen des Messkanals.

DE 102006024355 A1 UPAB: 20080102 NOVELTY - The micro-fluidic arrangement for detecting chemical, biochemical molecules and/or particles contained in liquid samples, comprises measuring channel (1) for the flow of the liquid, barrier elements (2) arranged on the measuring channel, and a heat exchanger. The barrier elements change the direction of flow of a part of the liquid sample, so that a part of the liquid sample does not flow parallelly to the longitudinal axis of the measuring channel. On bottom of the measuring channel a catcher molecules and/or catcher substances are immobilized in stripes shaped manner. DETAILED DESCRIPTION - The micro-fluidic arrangement for detecting chemical, biochemical molecules and/or particles contained in liquid samples, comprises measuring channel (1) for the flow of the liquid, barrier elements (2) arranged on the measuring channel, and a heat exchanger. The barrier elements change the direction of flow of a part of the liquid sample, so that a part of the liquid sample does not flow parallelly to the longitudinal axis of the measuring channel. On bottom of the measuring channel a catcher molecules and/or catcher substances are immobilized in stripes shaped manner. The barrier elements are arranged in an interval in an angle of 1-90degrees in reference to the longitudinal axis of the measuring channel and are not led across the entire width of the measuring channel. Between the barrier elements and the bottom of the measuring channel, a free gap, by which a part of the liquid sample flows, is trained. A heated liquid is led through the heat exchanger. The heat exchanger is formed with a Peltier element or controllable heating element. The measuring channel is sealed by a vacuum seal. The detection takes place optically and mass sensitively. Surface plasmones are stimulated during the detection. The arrangement keeps a constant internal pressure. The barrier elements alternately coming from the side edge of the measuring channel, are built and a front side end of barrier elements is arranged at the opposite side edge in a distance, so that a part of the liquid sample flows through the measuring channel with constantly changing direction of flow or flows through a meander shaped manner. USE - For detecting chemical, biochemical molecules and/or particles contained in liquid samples (claimed) such a rinsing liquid or buffer solution. ADVANTAGE - The arrangement achieves a high binding rate of analytes according to preimmobilized specific captures, thus avoiding measurement error and increasing sensitivity and safety of the analysis. This arrangement requires small volume of liquid samples, thus leading reduction of costs.