Tolerance induction or immunosuppression to prevent in particular Graft-versus-Host-Disease (GvHD) by short term pre-incubation of transplanted cell suspensions, tissues or organs coated with ligands to cell surface molecules

The present invention relates to, among others, an in vitro method of modifying a cell graft containing immune cells comprising the steps of incubating a cell graft containing immune cells with an anti CD4 antibody wherein said incubating is carried out for from 1 minute to 7 days, b) removing unbound antibody from said graft; as well as to corresponding modified grafts and uses.