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  4. Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices
 
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2018
Journal Article
Title

Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

Abstract
We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays.
Author(s)
Rizzo, Riccardo
Politecnico di Torino
Alvaro, Maria
Universität Turin
Danz, Norbert  
Fraunhofer-Institut für Angewandte Optik und Feinmechanik IOF  
Napione, Lucia
Universität Turin
Descrovi, Emiliano
Politecnico di Torino
Schmieder, Stefan
Fraunhofer-Institut für Werkstoff- und Strahltechnik IWS  
Sinibaldi, Alberto
Universität La Sapienza
Chandrawati, Rona
Imperial College London
Rana, Subinoy
Imperial College London
Munzert, Peter  
Fraunhofer-Institut für Angewandte Optik und Feinmechanik IOF  
Schubert, Thomas
KDS Radeberger Präzisions-Formen und Werkzeugbau GmbH
Maillart, Emmanuel
Scientific - HORIBA
Anopchenko, Aleksei
Universität La Sapienza
Rivolo, Paola
Politecnico di Torino
Mascioletti, Alessandro
LABOR Srl
Sonntag, Frank  orcid-logo
Fraunhofer-Institut für Werkstoff- und Strahltechnik IWS  
Stevens, Molly M.
Imperial College London
Bussolino, Federico
Universität Turin
Michelotti, Francesco
Universität La Sapienza
Journal
Sensors and Actuators. B  
Project(s)
BILOBA  
Funder
European Commission EC  
Open Access
DOI
10.1016/j.snb.2017.09.018
Additional full text version
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Language
English
Fraunhofer-Institut für Angewandte Optik und Feinmechanik IOF  
Fraunhofer-Institut für Werkstoff- und Strahltechnik IWS  
Keyword(s)
  • Biosensor platform

  • Bloch surface wave

  • Label-free optical sensor

  • Enhanced fluorescence

  • Tumor biomarker

  • Complex matrice

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