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Application of diffractive optical elements in confocal microscopy

: Li, Zheng

Fulltext urn:nbn:de:0011-n-5521913 (15 MByte PDF)
MD5 Fingerprint: 24db7984d5a6bc022089a34dfdf0fde5
Created on: 19.7.2019

Beyerer, Jürgen (Ed.); Taphanel, Miro (Ed.); Taphanel, Miro (Ed.):
Proceedings of the 2018 Joint Workshop of Fraunhofer IOSB and Institute for Anthropomatics, Vision and Fusion Laboratory
Karlsruhe: KIT Scientific Publishing, 2019 (Karlsruher Schriften zur Anthropomatik 40)
ISBN: 978-3-7315-0936-3
ISBN: 3-7315-0936-9
DOI: 10.5445/KSP/1000094782
Fraunhofer Institute of Optronics, System Technologies and Image Exploitation and Institute for Anthropomatics, Vision and Fusion Laboratory (Joint Workshop) <2018, Triberg-Nussbach>
Conference Paper, Electronic Publication
Fraunhofer IOSB ()

In traditional confocal microscopy, there is a tradeoff between the spatial resolution and the field of view due to the limitations of the objective lenses. To solve this problem, diffractive optical elements (DOEs) are used to generate illumination spots with high NA in a large area simultaneously. However, such DOEs in current research are only used as illuminators. In this work, the idea of superposition is utilized in DOEs to have flexible functionality and replace high-NA objectives for confocal measurement. To design the DOEs, different numerical simulation methods for light propagation are investigated and compared. Rayleigh-Sommerfeld integral is chosen to simulate the DOEs to get accurate results. Two kinds of DOEs are designed and simulated for 3D confocal surface measurements.