CC BY-NC-ND 4.0Gepp, M.M.GeppDuckstein, RowenaRowenaDucksteinKayatz, FabianFabianKayatzRodler, NorbertNorbertRodlerScheuerer, ZuzanaZuzanaScheuererNeubauer, Julia C.Julia C.NeubauerLachmann, KristinaKristinaLachmannStramm, CorneliaCorneliaStrammLiebmann, AndreaAndreaLiebmannThomas, MichaelMichaelThomasZimmermann, HeikoHeikoZimmermann2022-03-055.12.20172017https://publica.fraunhofer.de/handle/publica/25061410.24406/publica-r-25061410.1515/cdbme-2017-0077Novel approaches in regenerative medicine and tissue engineering are highly promoted by human induced pluripotent stem cells. Typical workflows include the expansion, differentiation and/or cryopreservation of these cells. Often, the differentiation, cytotoxicity tests or disease modelling rely on multicellular aggregates. Here, we present the novel bag-based system ""Labbag®"" to address workflows required for these applications. As a unique feature, the Labbag® provides a chemical spot pattern to allow the formation of several hundreds of hanging droplets within a few seconds with standard laboratory equipment. In this study, we describe the general concept of the Labbag® and show aggregate formation of stem cells as first stem cell workflow successfully transferred.enhanging dropletaggregatespheroidorganoidhuman pluripotent stem cellscell based modeljournal article