Enckevort, L.J.G. vanL.J.G. vanEnckevortDroc, G.G.DrocPiffanelli, P.P.PiffanelliGreco, R.R.GrecoGagneur, C.C.GagneurWeber, C.C.WeberGonzalez, V.M.V.M.GonzalezCabot, P.P.CabotFornara, F.F.FornaraBerri, S.S.BerriMiro, B.B.MiroLan, P.P.LanRafel, M.M.RafelCapell, T.T.CapellPuigdomenech, P.P.PuigdomenechOuwerkerk, P.B.F.P.B.F.OuwerkerkMeijer, A.H.A.H.MeijerPe', E.E.Pe'Colombo, L.L.ColomboChristou, P.P.ChristouGuiderdoni, E.E.GuiderdoniPereira, A.A.Pereira2022-03-032022-03-032005https://publica.fraunhofer.de/handle/publica/20964510.1007/s11103-005-8532-6A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs together with the original T-DNAs were assigned a position in the rice genome sequence represented as assembled pseudomolecules, and found to be distributed evenly over the entire rice genome with a distinct bias for predicted gene-rich regions. The bias of the Ds and Ac transposon inserts for genes was exemplified by the presence of 59% of the inserts in genes annotated on the rice chromosomes and 41% present in genes transcribed as disclosed by their homology to cDNA clones. In a screen for inserts in a set of 75 well annotated transcription factors, including homeobox-containing genes, we found six Ac/Ds inserts. This high frequency of Ds and Ac inserts in genes suggests that saturated knockout mutagenesis in rice using this strategy will be efficient and possible with a lower number of inserts than expected. These FSTs and the corresponding plant lines are publicly available through OrygenesDB database and from the EU consortium members.en570610620660572journal article