Spehar-Délèze, Anna-MariaAnna-MariaSpehar-DélèzeJulich, SandraSandraJulichGransee, RainerRainerGranseeTomaso, HerbertHerbertTomasoDulay, Samuel B.Samuel B.DulayO'Sullivan, Ciara K.Ciara K.O'Sullivan2022-03-052022-03-052016https://publica.fraunhofer.de/handle/publica/24414310.1007/s00216-016-9658-xAn electrochemiluminescence (ECL) immunosensor for the rapid detection of the Francisella tularensis pathogen using whole antibodies or antibody fragments as capture biomolecule is described. A sandwich immunoassay was used with either lipopolysaccharide (LPS) or the whole inactivated bacterial cell (LVS) as a target, while Ru(bpy)3 2+-encapsulated silicate nanoparticles were linked to the secondary antibody and used as ECL labels. The assay was performed in a fluidic chip housed in a custom-built black box incorporating electronics, optics and fluidics. The obtained limit of detection for LPS was 0.4 ng/mL, while for the LVS it was 70 and 45 bacteria/mL when the capturing molecule was the whole antibody and the antibody F(ab) fragment, respectively.enElectrochemilunescenceimmunoassayimmunosensorantibody fragmentbiowarfare agentscree-printed gold arraygole electrode array543journal article