Schleicher, M.M.SchleicherHansmann, J.J.HansmannElkin, B.B.ElkinKluger, Petra JulianePetra JulianeKlugerLiebscher, S.S.LiebscherHuber, A.J.T.A.J.T.HuberFritze, O.O.FritzeSchille, C.C.SchilleMüller, MichaelaMichaelaMüllerSchenke-Layland, KatjaKatjaSchenke-LaylandSeifert, M.M.SeifertWalles, HeikeHeikeWallesWendel, H.-P.H.-P.WendelStock, U.A.U.A.Stock2022-03-042022-03-042012https://publica.fraunhofer.de/handle/publica/22897010.1155/2012/397813In vivo self-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the creation of cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces. DNA-oligonucleotides immobilized by adsorption on parylene (poly(monoaminomethyl-para-xylene)) coated polystyrene and ePTFE were resistant to high shear stress (9.5N/m 2) and human blood serum for up to 96h. Adhesion of murine endothelial progenitor cells, HUVECs and endothelial cells from human adult saphenous veins as well as viability over a period of 14 days of HUVECs on oligonucleotide coated samples under dynamic culture conditions was significantly enhanced (P<0.05). Oligonucleotide-coated surfaces revealed low thrombogenicity and excellent hemocompatibility after incubation with human blood. These properties suggest the suitability of immobilization of DNA-oligonucleotides for biofunctionalization of blood vessel substitutes for improved in vivo endothelialization.en660journal article