Buyel, J.F.J.F.Buyel2022-03-062022-03-062019https://publica.fraunhofer.de/handle/publica/25885110.1007/s12268-019-1026-xPlants can be an alternative to mammalian cell cultures in terms of vaccine production due to low upstream production costs and a good safety profile. However, product purification can be challenging because of abundant host cell proteins (HCPs) in raw plant extracts. Here we report the optimization of blanching, a heat-based method, that allowed to remove over 90 percent of HCPs and to stabilize a malaria vaccine candidate by carefully adjusting the temperature in the step and combined it with solid-liquid separation and chromatographic purification.de540570571572journal article