Pudlas, M.M.PudlasKoch, S.S.KochBolwien, C.C.BolwienHirth, T.T.HirthWalles, H.H.WallesSchenke-Layland, K.K.Schenke-Layland2022-03-112022-03-112011https://publica.fraunhofer.de/handle/publica/37125110.1117/12.873047Bone-marrow mesenchymal stem cells (BM-MSCs) are a promising cell source for regenerative medicine. However, it is challenging to determine whether isolated BM-MSC populations are free of fibroblasts. We employed traditional methods and Raman spectroscopy to distinguish BM-MSCs and human dermal fibroblasts (hDFs). Although in vitro differentiation assays revealed the multipotent character of BM-MSCs, long culture periods are a major disadvantage. Using Raman spectroscopy, we could quickly distinguish between BM-MSCs and hDFs. Therefore we conclude that this method is sufficient for the rapid detection of fibroblastic contaminations in BM-MSC cultures.enbiomedical optical imagingbonecellular biophysicsRaman spectroscopy621conference paper