Sachse, RitaRitaSachseQuast, Robert B.Robert B.QuastSonnabend, AndreiAndreiSonnabendStech, MarlittMarlittStechKubick, StefanStefanKubick2022-03-042022-03-042014https://publica.fraunhofer.de/handle/publica/23691310.1007/s12268-014-0481-7Difficult to express membrane proteins represent an increasing amount of therapeutic molecules. Considerable optimization is often required for downstream applications such as assay development and functional characterization. Cell-free systems emerged as powerful tools for the synthesis of structurally and functionally divergent membrane proteins. Vesicle-based eukaryotic cell-free systems enable co-translational protein translocation and posttranslational modifications. Hence, these systems provide a multitude of options for membrane protein studies.dezellfreie ProteinsyntheseMembranproteinsynthesenicht-kanonische Aminosäure610620journal article