Koshkina, OlgaOlgaKoshkinaWestmeier, DanaDanaWestmeierLang, ThomasThomasLangBantz, ChristophChristophBantzHahlbrock, AngelinaAngelinaHahlbrockWürth, ChristianChristianWürthResch-Genger, UteUteResch-GengerBraun, UlrikeUlrikeBraunWeise, ChristophChristophWeiseThiermann, RaphaelRaphaelThiermannEravci, MuratMuratEravciMohr, BenjaminBenjaminMohrSchlaad, HelmutHelmutSchlaadStauber, Roland H.Roland H.StauberDocter, DominicDominicDocterBertin, AnnabelleAnnabelleBertinMaskos, MichaelMichaelMaskos2022-03-052022-03-052016https://publica.fraunhofer.de/handle/publica/24415110.1002/mabi.201600074Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.encellular uptakenanoparticlespoly(2-ethyl-2-oxazoline)poly(ethylene glycol)protein adsorption572journal article