Grumaz, ChristianChristianGrumazKirstahler, PhilippPhilippKirstahlerSohn, KaiKaiSohn2022-03-052022-03-052017https://publica.fraunhofer.de/handle/publica/24740910.1007/978-1-4939-6515-1_21Sequencing the whole genome of an organism is invaluable for its comprehensive molecular characterization and has been drastically facilitated by the advent of high-throughput sequencing techniques. Especially in clinical microbiology the impact of sequenced strains increases as resistance and virulence markers can easily be detected. Here, we describe a combined approach for sequencing a fungal genome and transcriptome from initial nucleic acid isolation through the generation of ready-to-load DNA libraries for the Illumina platform and the final step of genome assembly with subsequent gene annotation.ennext-generation sequencing (NGS)whole-genome shotgun sequencing (WGS)RNA sequencing (RNA-seq)Fungal genomeassemblyannotationBioinformaticsbook article