Elgeti, Vanessa KimberlyVanessa KimberlyElgetiSonntag, MirkoMirkoSonntagSohn, KaiKaiSohnJenner, LuccaLuccaJenner2026-02-052026-02-052025-03-12https://publica.fraunhofer.de/handle/publica/505707The present invention relates to a method for providing at least one detection template for detecting a marker sequence in a sample comprising template DNA, said method comprising (a) providing a plurality of elongation templates having a stem-loop structure from said template DNA; (b) providing at least one oligonucleotide (fusion primer) comprising a 5' universal sequence and a 3' targeting sequence; (c) incubating said plurality of elongation templates of step (a) with the least one fusion primer of step (b) under conditions (i) allowing hybridization of said at least one fusion primer to at least one target nucleic acid sequence in an elongation template, if present, and (ii) causing elongation along the elongation template of any fusion primer hybridized to an elongation template; (d) removing the fusion primer from the reaction products of step (c); and (e) thereby providing a detection template for detecting a marker sequence in a sample. The present invention also relates to a method for determining at least one marker sequence in a sample and to a method for diagnosing disease in a sample of a subject, as well as compositions of matter, devices, and kits related thereto.enpatentEP4520840 A1EP20230195471