Jamsari, A.A.JamsariNitz, I.I.NitzReamon-Büttner, S.M.S.M.Reamon-BüttnerJung, C.C.Jung2022-03-032022-03-032004https://publica.fraunhofer.de/handle/publica/20756310.1007/s00122-003-1529-02-s2.0-2142728612A HindIII BAC (bacterial artificial chromosome) library of asparagus (Asparagus officinalis L.) was established from a single male plant homozygous for the male flowering gene (MM). The library represents approximately 5.5 haploid genome equivalents with an average insert size of 82 kb. A subset of the library (2.6 haploid genome equivalents) was arranged into DNA pools. Using nine sex-linked amplified fragment length polymorphism (AFLP) and two sequence-tagged site (STS) markers, 13 different BAC clones were identified from this part of the library. The BACs were arranged into a first-generation physical map around the sex locus. Four PCR-derived markers were developed from the BAC ends, one of which could be scored in a co-dominant way. Using a mapping population of 802 plants we mapped the BAC-derived markers to the same position close to the M gene as the corresponding AFLP and STS markers. The markers are useful for further chromosome walking studies and as diagnos tic markers for selecting male plants homozygous for the M gene.en610576journal article