Inprakhon, PraneePraneeInprakhonWongthongdee, NatchaNatchaWongthongdeeAmornsakchai, TaweechaiTaweechaiAmornsakchaiPongtharankul, ThunyaratThunyaratPongtharankulSunintaboon, PanyaPanyaSunintaboonWiemann, Lars O.Lars O.WiemannDurand, AlainAlainDurandSieber, VolkerVolkerSieber2022-03-052022-03-052017https://publica.fraunhofer.de/handle/publica/25095010.1016/j.jbiotec.2017.07.021Sucrose monocaprate was synthesized by carrying out a lipase-catalyzed transesterification in a non-aqueous biphasic medium. Vinyl caprate was mechanically dispersed into a solution of sucrose in DMSO. The use of DMSO allowed increasing sucrose concentration up to 0.7. M (in DMSO). The denaturing effect of DMSO on lipase was avoided by pretreatment of lipase by pH adjustment in the presence of crown ether. This pretreatment maintained a significant catalytic activity which led to 0.2. M sucrose monoester within 1. h at 50. °C, which represented higher productivity than already reported. Detailed structural characterization revealed that only monoester was recovered and the 2-O-acylated sucrose monocaprate was the major isomer in the final product.enlipasesucrosesucrose estertransesterification660journal article