Kim, S.H.S.H.KimKim, E.-C.E.-C.KimKim, W.-J.W.-J.KimLee, M.-H.M.-H.LeeKim, S.-Y.S.-Y.KimKim, T.-J.T.-J.Kim2022-03-052022-03-052016https://publica.fraunhofer.de/handle/publica/24660710.1080/09168451.2016.1148574Angiogenesis, neovascularization from pre-existing vessels, is a key step in tumor growth and metastasis, and anti-angiogenic agents that can interfere with these essential steps of cancer development are a promising strategy for human cancer treatment. In this study, we characterized the anti-angiogenic effects of Coptis japonica Makino extract (CJME) and its mechanism of action. CJME significantly inhibited the proliferation, migration, and invasion of vascular endothelial growth factor (VEGF)-stimulated HUVECs. Furthermore, CJME suppressed VEGF-induced tube formation in vitro and VEGF-induced microvessel sprouting ex vivo. According to our study, CJME blocked VEGF-induced cell cycle transition in G1. CJME decreased expression of cell cycle-regulated proteins, including Cyclin D, Cyclin E, Cdk2, and Cdk4 in response to VEGF.en660572journal article