Berger-Preiß, E.E.Berger-PreißGerling, S.S.GerlingApel, E.E.ApelLampen, A.A.LampenCreutzenberg, O.O.Creutzenberg2022-03-042022-03-042010https://publica.fraunhofer.de/handle/publica/22198210.1007/s00216-010-3928-9We have developed a highly selective and sensitive method using gas chromatography-mass spectrometry with negative chemical ionization for measuring 3-chloropropane-1,2-diol (3-MCPD) in rat blood and urine. Samples were adsorbed on silica gel, extracted with ethyl acetate, and derivatized by chemical derivatization with heptafluorobutyric acid anhydride. For quantification, matrix-based calibration curves and 3-MCPD-d (5), as an isotope-labeled internal standard, were used. The relative recoveries of 3-MCPD were between 80 and 110% in most cases and the relative standard deviations were typically less than 10%, with some exceptions. The limit of quantification of the method was found to be about 2 ng/mL. In conclusion, a valuable, robust, and sensitive method for detection of 3-MCPD is now available for biokinetics studies.enbiomonitoring3-chloropropane-1,2-diolnegative chemical ionizationratbloodUrinegas chromatographymass spectrometry610620543journal article