Pallavi, PramaPramaPallaviScheuermann, StefanStefanScheuermann2024-04-232024-04-232024https://publica.fraunhofer.de/handle/publica/46676210.1007/978-1-0716-3621-3_12-s2.0-8518199111238194024Primary cells form the basis of modern-day in vitro research analysis tools. Many conventional procedures for generating single-cell suspensions from solid tissue are neither robust nor reproducible. Here we describe primary cells isolation from surgically resected tumor tissue via enzyme-free mechanical dissociation using TissueGrinder, a novel semi-automated benchtop device. The isolated cells can be used for any downstream biochemical or cell-based analytic assay.enEnzyme-free cell isolationMechanical dissociationPrimary cellsSingle-cell isolationbook article