Geißler, D.D.GeißlerHill, D.D.HillLöhmannsröben, H.-G.H.-G.LöhmannsröbenThomas, E.E.ThomasLavigne, A.A.LavigneDarbouret, B.B.DarbouretBois, E.E.BoisCharbonniere, L.J.L.J.CharbonniereZiessel, R.F.R.F.ZiesselHildebrandt, N.N.Hildebrandt2022-03-112022-03-112010https://publica.fraunhofer.de/handle/publica/36957810.1117/12.842731An optical multiplexed homogeneous (liquid phase) immunoassay based on FRET from a terbium complex to eight different fluorescent dyes is presented. We achieved highly sensitive parallel detection of four different lung cancer specific tumor markers (CEA, NSE, SCC and CYFRA21-1) within a single assay and show a proof-of-principle for 5- fold multiplexing. The method is well suited for fast and low-cost miniaturized point-of-care testing as well as for highthroughput screening in a broad range of in-vitro diagnostic applications.en668conference paper