Ickrath, PascalPascalIckrathIckrath, KatrinKatrinIckrathSteinke, MariaMariaSteinkeScherzad, AgmaAgmaScherzadKleinsasser, NorbertNorbertKleinsasserLodes, NinaNinaLodesBregenzer, MaximilianMaximilianBregenzerHagen, RudolfRudolfHagenHackenberg, StephanStephanHackenberg2022-03-062022-03-062020https://publica.fraunhofer.de/handle/publica/26645210.1089/ten.tea.2020.0089For culture models of primary cells of the human nasal mucosa, monocultures with epithelial cells (ECs) are used as well as cocultures with ECs and fibroblasts (FBs). Well-differentiated models of the respiratory nasal epithelium can be used for ecogenotoxicological assessments, for experiments on host/pathogen interactions, or tissue engineering. However, long-term cultivation and repeated passaging may induce a loss of DNA integrity or cell functionality. The aim of this study was to evaluate these parameters in test systems created from primary nasal mucosa cells. Enzymatic and sequential cell isolation from nasal tissue was performed. EC monocultures and compartment-separated EC-FB cocultures were cultivated over three passages under air/ liquid interface conditions. DNA stability and regenerative capacity at the DNA and chromosomal level as well as proliferation and cell differentiation were examined. Both methods showed equivalent levels of DNA stability and regenerative capacity over all passages. Sequential growth of the coculture provided higher cell purity, while enzymatic cell harvest was associated with FB contamination in EC culture. Mucociliary differentiation was verified with electron microscopy in both methods. Functionality measured by lipopolysaccharide stimulation of interleukins was constant over long-term cultivation. Our data confirm DNA stability in long-term cell cultivation as well as functional integrity in both culture methods. Sequential cell isolation should be favored over enzymatic isolation due to higher culture purity.encocultureDNA stabilityepithelial cell modelshuman nasal mucosatissue system666journal article