Gottschall, ThomasThomasGottschallMeyer-Zedler, TobiasTobiasMeyer-ZedlerEibl, MatthiasMatthiasEiblPfeiffer, TomTomPfeifferHakert, HubertusHubertusHakertSchmitt, MichaelMichaelSchmittHuber, RobertRobertHuberTünnermann, AndreasAndreasTünnermannLimpert, JensJensLimpertPopp, JuergenJuergenPopp2023-03-282023-03-282023https://publica.fraunhofer.de/handle/publica/43923610.1021/acs.jpcb.2c09115Coherent Raman scattering microscopy utilizing bioorthogonal tagging approaches like isotope or alkyne labeling allows for a targeted monitoring of spatial distribution and dynamics of small molecules of interest in cells, tissues, and other complex biological matrices. To fully exploit this approach in terms of real-time monitoring of several Raman tags, e.g., to study drug uptake dynamics, extremely fast tunable lasers are needed. Here, we present a laser concept without moving parts and fully electronically controlled for the quasi-simultaneous acquisition of coherent anti-Stokes Raman scattering images at multiple Raman resonances. The laser concept is based on the combination of a low noise and spectrally narrow Fourier domain mode-locked laser seeding a compact four wave mixing-based high-power fiber-based optical parametric amplifier.enCoherent scatteringFiber amplifiersFiber lasersFibersFour wave mixingjournal article